The group said that such charges were not new under Morsi, who was elected last June after the 2011 overthrow of longtime leader Hosni Mubarak, but were becoming more frequent.
While Youssef's fast-paced show has attracted a wide viewership, it also has earned a fair share of detractors. Youssef has been a frequent target of lawsuits, most of them brought by Islamist lawyers who have accused him of "corrupting morals" or violating "religious principles."
Recent legal moves against protesters, activists and critics come as unrest in Egypt continues amid deep political polarization.
The opposition charges that Morsi, in office for nine months, has failed to tackle any of the nation's most pressing problems. They say the Brotherhood is trying to monopolize power, breaking its promise to include other factions in key decisions.
Morsi blames the country's woes on corruption under Mubarak as well as ongoing protests. He says the opposition has no grassroots support and, along with former regime supporters, is stoking unrest for political gain.