Wallace earlier pleaded not guilty to a charge of accessory to murder after the fact. Ortiz pleaded not guilty to the firearm charge. A judge ordered Wallace held on $500,000 bail and Ortiz held without bail. Singleton's family hasn't commented.
Authorities have said they haven't found the murder weapon, which they believe was a .45-caliber Glock pistol.
But they said they recovered a magazine for .45-caliber bullets in Hernandez's Hummer and ammunition of the same caliber inside a condo he rented in Franklin.
Prosecutors say they have surveillance video from Hernandez's home showing him before and after Lloyd's killing holding what appears to be a Glock. Authorities say they've also recovered a shell casing that matched those found at the homicide scene after tracking the rental car Hernandez was in the night Lloyd died.
Since then, Boston police have asked police in Hernandez's hometown, Bristol, Conn., for their help with the probe into Lloyd's homicide and a 2012 double homicide near a Boston nightclub. A Connecticut police lieutenant said authorities searched the home of Hernandez's uncle, seizing an SUV sought in the double killing that had been rented in Hernandez's name.
Two men died in the July 2012 shooting in Boston's South End, with witnesses reporting that gunfire came from inside a gray SUV with Rhode Island tags. Boston police haven't reported any arrests in the deaths of Daniel Jorge Correia de Abreu and Safiro Teixeira Furtado and won't comment on whether Hernandez is a suspect.
Hernandez also faces civil litigation after a Connecticut man filed a lawsuit asserting the former player shot him in the face in February after they argued at a Miami strip club. Alexander Bradley, who says he lost an eye, told police at the time he didn't know who shot him.
Also Thursday, Hernandez's attorneys asked an Attleboro judge to order the state to stop "deliberately misleading or making false statements" to potential witnesses. They claim detectives visiting a potential witness at a Connecticut prison to interview him told the man this month they were there "to help Aaron out."
Sutter called it an allegation and said he hadn't reviewed the defense motion.
Associated Press writers Bridget Murphy in Boston and Michelle R. Smith in Fall River contributed to this report.