Center fielder Aaron Hicks had a hit and an RBI and became the first Twin to play all nine innings in spring training.
"He'll probably ask for a couple of days off now," manager Ron Gardenhire said.
Miami's Kevin Kouzmanoff hit a two-run homer. Giancarlo Stanton went 0 for 3 with a sacrifice fly in his final game before joining the U.S. team for the WBC.
The game was a reunion for former Twins catcher Mike Redmond, the Marlins' new manager. Gardenhire was glad to see his protege get the job.
"When he would be on the bench, we'd talk," Gardenhire recalled.
"'Would you hit-and-run here?' 'Absolutely.'
"'Would you bunt him over here?' 'Absolutely.'
"I'd say, 'Well then, you do the interviews when he screws it up.' And now he gets that chance."